2JAY
Proteasome beta subunit PrcB from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.504, 40.473, 59.937 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 59.440 - 1.990 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fhg |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.496 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.010 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.080 | 0.290 |
| Number of reflections | 13316 | |
| <I/σ(I)> | 18 | 4.2 |
| Completeness [%] | 98.3 | 85.7 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M AMMONIUM SULFATE 30% PEG 4000 |
