2JAF

Ground state of halorhodopsin T203V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	94.473, 94.473, 157.137
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.238
Rwork	0.238
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e12
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.600
Rmerge	0.140	0.740
Number of reflections	35303
<I/σ(I)>	24.9	2.4
Completeness [%]	95.9	89.5
Redundancy	8	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE			CRYSTALLIZED IN A CUBIC LIPID PHASE MADE OF 58-62 W/V % 1-MONOOLEIN, 3.3 M KCL, 3.5 MG/ML HR AND 25 MM TRIS/HCL, PH 7, R.T.