2J8X

Epstein-Barr virus uracil-DNA glycosylase in complex with Ugi from PBS-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-20
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	62.406, 82.942, 269.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.880 - 2.300
R-factor	0.196
Rwork	0.193
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lqm
RMSD bond length	0.004
RMSD bond angle	0.829
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.300	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.100	0.350
Number of reflections	29123
<I/σ(I)>	5.4	2.16
Completeness [%]	92.3	90.5
Redundancy	5.81	5.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		HANGING DROP VAPOUR DIFFUSION METHOD. PROTEIN IN 100 MM NACL, 20 MM TRIS-HCL PH 7.5 AND 10 MM DTT AT 30 TO 50 MG/ML. RESERVOIR SOLUTION OF 20% PEG 3350 AND 0.05 M NH4CL.