2J8Q
Crystal structure of human cleavage and polyadenylation specificity factor 5 (CPSF5) in complex with a sulphate ion.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-04-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.400, 86.400, 139.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cl3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.500 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.320 |
| Number of reflections | 24012 | |
| <I/σ(I)> | 13.6 | 3.94 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17%(W/V)PEG3350, 0.2M AMSO4, 0.1M BIS TRIS PH 5.2.MIX 1:1UL DROPS OF 9MG/ML PROTEIN WITH WELL SOLUTION IN RT THEN MOVE TO CR. |
