2J8I
Structure of NP275, a pentapeptide repeat protein from Nostoc punctiforme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 194 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.181, 62.558, 100.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.500 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.500 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.170 |
| Number of reflections | 11358 | |
| <I/σ(I)> | 23.6 | 8.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 30% PEG3350 100 MM NACACODYLATE PH 6.8 200MM LICL2 PROTEIN 25MG/ML |
