2J8G
Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (E94Q mutant in complex with a tetrasaccharide- pentapeptide)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.520, 97.390, 127.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.250 - 1.690 |
| R-factor | 0.18656 |
| Rwork | 0.187 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h09 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.496 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.620 | 1.810 |
| High resolution limit [Å] | 1.510 | 1.690 |
| Rmerge | 0.120 | 0.500 |
| Number of reflections | 58301 | |
| <I/σ(I)> | 3.3 | 1.1 |
| Completeness [%] | 92.6 | 71.4 |
| Redundancy | 5.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
