2J8D
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 8 in the charge-separated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-02 |
| Detector | MARRESERACH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 140.010, 140.010, 184.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 119.520 - 2.070 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.545 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.520 | 2.100 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 123115 | |
| <I/σ(I)> | 10.84 | 2.22 |
| Completeness [%] | 95.6 | 95 |
| Redundancy | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
