2J8A
X-ray structure of the N-terminus RRM domain of Set1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 123.790, 123.790, 123.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.27700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.054 |
| RMSD bond angle | 4.097 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.010 | 0.310 |
| Number of reflections | 7192 | |
| <I/σ(I)> | 23.8 | 6.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.4 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 2.15M AMMONIUM SULFATE,50MM NACITRATE PH4.6,4% POLYPROPYLENE-GLYCOL 400,3% GLYCEROL, pH 4.60 |
