2J82
Structural analysis of the PP2C Family Phosphatase tPphA from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95,0.9796,1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.216, 151.799, 82.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.810 - 1.280 |
| R-factor | 0.1633 |
| R-free | 0.20420 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.810 | 1.370 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.020 | 0.260 |
| Number of reflections | 61884 | |
| <I/σ(I)> | 25.01 | 3.32 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 0.99 | 0.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M CACL2, 0.1 M HEPES PH 8.0, 28% PEG 3350 |
