2J74

Structure of Beta-1,4-Galactanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-14
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	50.430, 80.250, 104.500
Unit cell angles	90.00, 99.50, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.2025
Rwork	0.203
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r8l
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.340
Number of reflections	25348
Completeness [%]	100.0	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				30-35%(W/V) PEG1500