2J6H
E. coli glucosamine-6-P synthase in complex with glucose-6P and 5-oxo- L-norleucine
Replaces: 2BPJExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.300, 91.230, 184.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.350 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1GDO AND 1MOQ |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.807 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 57253 | |
| <I/σ(I)> | 9.55 | 2.09 |
| Completeness [%] | 96.0 | 83 |
| Redundancy | 3.11 | 2.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 12% PEG 8000, 0.1 M KCL, 5% GLYCEROL, 10 MM FRUCTOSE-6-PHOSPHATE., pH 7.20 |
