2J6G
FaeG from F4ac ETEC strain 5_95, produced in tobacco plant chloroplast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.335, 57.575, 69.900 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.228 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 41286 | |
| <I/σ(I)> | 11.1 | 3.33 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M SODIUM CACODYLATE PH=6.5, 1.4M SODIUM ACETATE TRIHYDRATE |
