2J6A

Structure of S. cerevisiae Trm112 protein, a methyltransferase activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-24
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	92.600, 38.460, 45.810
Unit cell angles	90.00, 102.24, 90.00

Refinement procedure

Resolution	19.670 - 1.700
R-factor	0.2
Rwork	0.198
R-free	0.23600
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.296
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.130	0.520
Number of reflections	17182
<I/σ(I)>	6.2	2.4
Completeness [%]	97.7	99
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		1.2M KH2PO4 PH 7 PROTEIN CONCENTRATION 10MG/ML IN 10 MM TRIS-HCL PH 8, 10UM ZNCL2, 6MM B-MERCAPTOETHANOL