2J68

Bacterial dynamin-like protein BDLP, GDP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	I 2 2 2
Unit cell lengths	77.586, 154.950, 247.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.2231
Rwork	0.223
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j69
RMSD bond length	0.007
RMSD bond angle	1.259
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.070	0.330
Number of reflections	26584
<I/σ(I)>	15.6	3.3
Completeness [%]	99.6	99.6
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1