2J57
X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- quinol in complex with amicyanin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.131, 123.439, 246.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 2.250 |
| R-factor | 0.183 |
| Rwork | 0.179 |
| R-free | 0.24100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.981 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.200 | 0.470 |
| Number of reflections | 162354 | |
| <I/σ(I)> | 4.4 | 2.2 |
| Completeness [%] | 97.1 | 95.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | pH 5.60 |
