2J44
Alpha-glucan binding by a streptococcal virulence factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.555, 89.124, 59.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.225 |
| Rwork | 0.221 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j43 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.554 |
| Phasing software | SPYD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.510 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 15873 | |
| <I/σ(I)> | 10.3 | 3.1 |
| Completeness [%] | 96.9 | 99.1 |
| Redundancy | 4.4 | 4.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
