2J1O
Geranylgeranyl diphosphate synthase from Sinapis alba
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.810, 71.810, 127.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.23000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.563 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 26607 | |
| <I/σ(I)> | 17.2 | 5.5 |
| Completeness [%] | 99.1 | 96.5 |
| Redundancy | 8.5 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | pH 10.00 |
