2J0R
Structure of the haem-chaperone Proteobacteria-protein HemS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2006-03-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.022, 68.269, 73.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.060 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j0p |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.367 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.350 |
| Number of reflections | 25260 | |
| <I/σ(I)> | 17.9 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM BICINE PH 9, 2.45M AMMONIUM SULPHATE, 5% PEG 400 |
