2J0K
Crystal structure of a fragment of focal adhesion kinase containing the FERM and kinase domains.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-20 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.022, 106.209, 240.881 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.490 - 3.000 |
R-factor | 0.237 |
Rwork | 0.234 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1MP8 AND 2AEH |
RMSD bond length | 0.011 |
RMSD bond angle | 1.333 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.100 | 0.420 |
Number of reflections | 32779 | |
<I/σ(I)> | 16.57 | 2.13 |
Completeness [%] | 96.7 | 70.9 |
Redundancy | 6.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 15% PEG10K, 300MM NACL, 100MM TRIS PH8.5, 10MM TCEP, pH 8.50 |