2J0B

Structure of the catalytic domain of mouse Manic Fringe in complex with UDP and manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	161.760, 40.970, 38.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.850 - 2.100
R-factor	0.18
Rwork	0.177
R-free	0.22100
Structure solution method	OTHER
RMSD bond length	0.019
RMSD bond angle	1.773
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.600	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.105	0.464
Number of reflections	15588
<I/σ(I)>	13.6	4.4
Completeness [%]	99.6	100
Redundancy	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M POTASSIUM SULFATE, 20% PEG 3350, pH 6.50