2J05
Crystal structure of the RasGAP SH3 domain at 1.5 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 32.800, 32.800, 183.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.410 - 1.500 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 35348 | |
| <I/σ(I)> | 20.2 | 7.3 |
| Completeness [%] | 98.4 | 89.2 |
| Redundancy | 6.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 100 MM KNO3, 100 MM TAPS, PH 9 AND 40% PEG 8000 |
