2J04
The tau60-tau91 subcomplex of yeast transcription factor IIIC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.420, 125.800, 210.470 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 208.510 - 3.200 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.754 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 49476 | |
| <I/σ(I)> | 5.4 | 1.8 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.8 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.0 |
