2IZX
Molecular Basis of AKAP Specificity for PKA Regulatory Subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.352, 45.251, 56.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.420 - 1.300 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RII APO CRYSTAL STRUCTURE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.805 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 26863 | |
| <I/σ(I)> | 16 | 2.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.35 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1 M TRI-SODIUM CITRATE, 0.1 M TRIS-HCL PH 7.5 |
