2IZW
Crystal structure of Ryegrass Mottle Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 277.641, 298.719, 392.485 |
| Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
| Resolution | 34.900 - 2.900 |
| R-factor | 0.294 |
| Rwork | 0.294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ng0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | AVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.170 | |
| Number of reflections | 569869 | |
| <I/σ(I)> | 5 | 1.06 |
| Completeness [%] | 40.5 | 22.9 |
| Redundancy | 1.5 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.3M LITHIUM SULPHATE, 0.05 M NATRIUM CITRATE, PH 3.0, 1% PEG 8000, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 296K |
