2IZS
Structure of casein kinase gamma 3 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-10 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.029, 56.029, 220.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.260 - 1.950 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2chl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 29640 | |
| <I/σ(I)> | 11 | 1.9 |
| Completeness [%] | 97.4 | 83.2 |
| Redundancy | 4.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% PEG10K, 0.20 M MGCL2, 0.10 M TRIS PH=7.0, pH 7.00 |
