2IZP
BipD - an invasion protein associated with the type-III secretion system of Burkholderia pseudomallei.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.509, 56.204, 84.208 |
| Unit cell angles | 90.00, 94.47, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.226 |
| R-free | 0.29900 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.017 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 104464 | |
| <I/σ(I)> | 10.4 | 2.8 |
| Completeness [%] | 97.5 | 93.4 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | [BIPD]=4 MG/ML, 20-25% PEG 4000, 5MM NICKEL CHLORIDE, 0.1M GLYCINE, 0.1M SODIUM CACODYLATE PH 5.0-5.5. |
