2IYJ
Crystal structure of the N-terminal dimer domain of E.coli DsbC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-03-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 42.138, 42.138, 268.677 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.770 - 2.000 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eej |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.770 | 2.070 |
| High resolution limit [Å] | 2.070 | 2.000 |
| Rmerge | 0.080 | 0.540 |
| Number of reflections | 10340 | |
| <I/σ(I)> | 36.6 | 3.6 |
| Completeness [%] | 97.2 | 84.4 |
| Redundancy | 12.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 2M LITHIUM SULPHATE,0.1M MAGNESIUM SULPHATE, 5%ISOPROPNOL (PH4.5), pH 4.50 |
