2IYE
Structure of catalytic CPx-ATPase domain CopB-B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.530, 53.800, 73.960 |
| Unit cell angles | 90.00, 97.08, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.224 |
| Rwork | 0.220 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.848 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 17386 | |
| <I/σ(I)> | 14.1 | 4.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG-6000 200 MM AMMONIUM SULFATE 10 MM DTT 100 MM SODIUM CACODYLATE, PH 6.5 |
