2IY1
SENP1 (mutant) full length SUMO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.232, 141.232, 98.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.720 - 2.460 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 34278 | |
| <I/σ(I)> | 14.7 | 2.1 |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
