2IXV

Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (E94Q mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-07-30
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	79.965, 96.443, 127.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.530 - 1.960
R-factor	0.223
Rwork	0.223
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h09
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.530	2.080
High resolution limit [Å]	1.960	1.960
Rmerge	0.080	0.400
Number of reflections	40900
<I/σ(I)>	5.1	1.7
Completeness [%]	99.4	100
Redundancy	4.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0