2IXO

CRYSTAL STRUCTURE OF THE PP2A PHOSPHATASE ACTIVATOR Ypa1 PTPA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	53.084, 94.178, 139.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.215
Rwork	0.211
R-free	0.29100
Structure solution method	OTHER
RMSD bond length	0.018
RMSD bond angle	1.841
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Rmerge	0.140
Number of reflections	21334
<I/σ(I)>	11.9
Completeness [%]	95.5
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		25% PEG400, 0.1M NACL AND 0.1M MES PH 5.6