2IXN

CRYSTAL STRUCTURE OF THE PP2A PHOSPHATASE ACTIVATOR Ypa2 PTPA2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	157.930, 171.042, 53.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.202
Rwork	0.199
R-free	0.27300
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.226
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.190
Number of reflections	176445
<I/σ(I)>	11.5
Completeness [%]	91.4
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		16% PEG4000, 0.4M KCL, PH 5.6, 0.1M TRIS-HCL PH 8.5