2IXB
Crystal structure of N-ACETYLGALACTOSAMINIDASE in complex with GalNAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 88.189, 88.189, 301.438 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ixa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 27626 | |
| <I/σ(I)> | 33.7 | 9.4 |
| Completeness [%] | 98.2 | 84.3 |
| Redundancy | 12.3 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.25 | 46-50% 2-METHYL-2,4-PENTANEDIOL (MPD), 75 MM NA-CITRATE PH 6.25 |
