2IXA
A-zyme, N-acetylgalactosaminidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 87.659, 87.659, 299.427 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.040 - 2.300 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.490 |
| Number of reflections | 30652 | |
| <I/σ(I)> | 25.7 | 4.5 |
| Completeness [%] | 98.0 | 82.8 |
| Redundancy | 11.3 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.25 | 46-50% 2-METHYL-2,4-PENTANEDIOL (MPD), 75 MM NA-CITRATE PH 6.25 |
