2IWT
Thioredoxin h2 (HvTrxh2) in a mixed disulfide complex with the target protein BASI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-07 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.148, 76.148, 129.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.910 - 2.300 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AVA AND UNPUBLISHED STRUCTURE OF BARLEY TRXH2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.650 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 16933 | |
| <I/σ(I)> | 6.6 | 1.7 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 4.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M CITRIC ACID PH 5.0, 12% PEG6000 |
