2IWK

Inhibitor-bound form of nitrous oxide reductase from Achromobacter Cycloclastes at 1.7 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	71.114, 120.913, 137.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.17
Rwork	0.168
R-free	0.20700
Structure solution method	OTHER
RMSD bond length	0.016
RMSD bond angle	1.587
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Rmerge	0.140
Number of reflections	128667
<I/σ(I)>	7.8
Completeness [%]	98.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1