2IWA
Unbound glutaminyl cyclotransferase from Carica papaya.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 97.740, 97.740, 64.974 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.040 - 1.600 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| R-free | 0.16800 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.533 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | XPREP |
| Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 46684 | |
| <I/σ(I)> | 18.2 | 5.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOUR DIFFUSION METHOD EMPLOYING CRYSCHEM-PLATES FROM EQUIVOLUMETRIC DROPS CONTAINING PROTEIN SOLUTION (46-52 MG/ML IN 10MM HEPES PH 7.0, 150MM NACL) AND 28-32% PEG4000, 0.2M MGCL2, 0.1M TRIS-HCL PH 8.5 AS PRECIPITATING SOLUTION. |
