2IW1
Crystal Structure of WaaG, a glycosyltransferase involved in lipopolysaccharide biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-04-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.718, 88.352, 89.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.500 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.680 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.130 | 0.280 |
| Number of reflections | 64367 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 99.0 | 93.6 |
| Redundancy | 4.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% PEG3350, 0.2M NABR, 0.1M MES PH 6.75 |
