2IVN
Structure of UP1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.933, 69.868, 75.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.987 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.510 |
| Number of reflections | 41853 | |
| <I/σ(I)> | 25.1 | 2.8 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 4.1 | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 25% PEG 4000, 0.3M KCL, 0.1M SODIUM CITRATE PH5.6 |
