2IV8
beta appendage in complex with b-arrestin peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.952, 35.484, 190.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 2.800 |
| R-factor | 0.22 |
| Rwork | 0.213 |
| R-free | 0.36900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e42 CHAIN A |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.246 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.780 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.150 |
| Number of reflections | 4759 | |
| <I/σ(I)> | 8.7 | 5 |
| Completeness [%] | 70.5 | 63.5 |
| Redundancy | 5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.2M MG FORMATE 30% PEG 3350 OTHER REMARKS: THIS WAS THE ONLY CRYSTAL OBTAINED DESPITE HEROIC ATTEMPTS. THE CRYSTAL WAS HIGHLY MOSAIC, AND EPITAXIALLY SPLIT, LIMITING THE AMOUNT OF DATA WHICH COULD BE COLLECTED., pH 7.40 |
