2IV0
Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.611, 65.405, 87.181 |
| Unit cell angles | 90.00, 95.28, 90.00 |
Refinement procedure
| Resolution | 86.710 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v94 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.785 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 31867 | |
| <I/σ(I)> | 11.1 | 3.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 0.6M ZNSO4, 0.1M NACACODYLATE, PH6.3, pH 6.30 |
