2IUU

P. aeruginosa FtsK motor domain, hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	140.012, 221.757, 134.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.900
R-factor	0.2334
Rwork	0.233
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.521
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.380
Number of reflections	90848
<I/σ(I)>	13.1	3.3
Completeness [%]	99.8	99.8
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1