2IUG
Crystal structure of the PI3-kinase p85 N-terminal SH2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.120, 57.120, 112.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.890 |
| R-factor | 0.1918 |
| Rwork | 0.192 |
| R-free | 0.28900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.671 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmerge | 0.050 | 0.190 |
| Number of reflections | 14679 | |
| <I/σ(I)> | 3.56 | |
| Completeness [%] | 95.1 | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG4000, 50MM MGCL2, 50MM TRIS PH7.5 |
