2IUC
Structure of alkaline phosphatase from the Antarctic bacterium TAB5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.043, 173.184, 55.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.740 - 1.950 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ed9 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.934 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.130 | 0.260 |
| Number of reflections | 48685 | |
| <I/σ(I)> | 12.3 | 2.3 |
| Completeness [%] | 97.3 | 84.3 |
| Redundancy | 4.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | HANGING-DROP, 4 DEGREE C, 17 MG/ML PROTEIN, 23% PEG3350, 0.2M NAAC, 10MM 4-NITROPHENYL PHOSPHATE, 0.1M CACODYLATE, PH 6.5 |
