2IU5
Dihydroxyacetone kinase operon activator DhaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.459, 46.284, 171.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 45492 | |
| <I/σ(I)> | 16 | 0.07 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 4.4 | 4.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | THE CONCENTRATED PROTEIN (20 MG/ML) WAS MIXED WITH BUFFER CONTAINING 50 MM NA-CACODYLATE PH 6.0, 1.7 M AMMONIUM SULFATE, 0.015 M MG-ACETATE IN A 1:1 RATIO. |
