2IU1
Crystal structure of eIF5 C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-01 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.137, 70.254, 81.705 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.188 |
Rwork | 0.185 |
R-free | 0.22100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.080 |
Number of reflections | 104040 | |
<I/σ(I)> | 27.6 | 8.5 |
Completeness [%] | 98.4 | 97.4 |
Redundancy | 5.9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 293 | 0.2 M MGCL2, 0.1 M TRIS PH 8.5, 25% PEG 3350, 20 DEG C |