2ITG
CATALYTIC DOMAIN OF HIV-1 INTEGRASE: ORDERED ACTIVE SITE IN THE F185H CONSTRUCT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-05 |
Detector | RIGAKU |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.400, 72.400, 65.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.2 * |
Rwork | 0.200 |
Structure solution method | MR |
Starting model (for MR) | 1itg |
RMSD bond length | 0.011 |
RMSD bond angle | 0.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.085 | 0.281 |
Total number of observations | 14682 * | |
Number of reflections | 5012 | |
<I/σ(I)> | 8.63 | 1.88 |
Completeness [%] | 79.8 | 54.4 |
Redundancy | 2.93 | 1.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | 19 * | 3-9% PEG 8000, 0.4M AMMONIUM SULFATE, 0.1M SODIUM CACODYLATE PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5-4 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 0.05 (M) | |
3 | 1 | drop | ammonium sulfate | 0.2 (M) | |
4 | 1 | drop | PEG8000 | 1.5-4.5 (%) | |
5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
6 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
7 | 1 | reservoir | PEG8000 | 3-9 (%) |