2ISV

Structure of Giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	62.360, 62.360, 319.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.199
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1JVF
RMSD bond length	0.018
RMSD bond angle	1.970
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.037	0.116
Number of reflections	28450
Completeness [%]	91.6	77.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.75	298	18-23% Polyethylglycol monomethyl ether 2000, 0.1 M Tris-HCl, 0.2 M MgCl2, 20 mM PGH, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K