2ISQ

Crystal Structure of O-Acetylserine Sulfhydrylase from Arabidopsis Thaliana in Complex with C-Terminal Peptide from Arabidopsis Serine Acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-15
Detector	BRUKER SMART 6000
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	104.860, 104.860, 99.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.800
Rwork	0.188
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1Z7W
RMSD bond length	0.006
RMSD bond angle	1.280
Data reduction software	SAINT
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	19828
<I/σ(I)>	7.36	2.01
Completeness [%]	100.0	100
Redundancy	10.8	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	1.5 M ammonium sulfate; 0.1 M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K