2ISN
Crystal structure of a phosphatase from a pathogenic strain Toxoplasma gondii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792, 0.9788,0.9500 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.986, 62.447, 63.738 |
| Unit cell angles | 65.53, 70.21, 77.64 |
Refinement procedure
| Resolution | 43.060 - 1.900 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 54775 | |
| <I/σ(I)> | 8.8 | 1 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 7.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 2M Li2So4, 0.5M Ammonium sulfate, 0.1M Na-citrate, ph 5.6, praseodymium acetate, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 2M Li2So4, 0.5M Ammonium sulfate, 0.1M Na-citrate, ph 5.6, praseodymium acetate, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 2M Li2So4, 0.5M Ammonium sulfate, 0.1M Na-citrate, ph 5.6, praseodymium acetate, VAPOR DIFFUSION, temperature 298K |
